This study aims to analyze the influence of organizational climate and job autonomy on harmonious work passion and its impact on the performance of lecturers at Politeknik Pertanian Negeri Samarinda. The study involved 98 lecturers selected using purposive sampling based on specific criteria. Data analysis was conducted using Structural Equation Modeling (SEM) with Smart PLS to assess both direct and indirect effects among variables. The results indicate that organizational climate has a significant positive effect on harmonious work passion, as does job autonomy. However, harmonious work passion does not significantly affect lecturer performance. Organizational climate shows a significant negative direct impact on performance, while job autonomy has no significant effect. The indirect influence through harmonious work passion is also not significant for both organizational climate and job autonomy on performance. These findings suggest that the harmonious work passion formed by organizational climate and job autonomy requires additional resources and support systems to enhance performance. The study concludes that a balanced approach between a conducive organizational climate and guided autonomy is essential for improving performance.